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JoVE Encyclopedia of Experiments
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10.3791/55812-v
12:53 min
Bidirectional Retroviral Integration Site PCR Methodology and Quantitative Data Analysis Workflow
10.3791/55903-v
11:35 min
Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
10.3791/56187-v
11:42 min
Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish
10.3791/56264-v
A Universal Protocol for Large-scale gRNA Library Production from any DNA Source
10.3791/56275-v
16:08 min
Microinjection of CRISPR/Cas9 Protein into Channel Catfish, Ictalurus punctatus, Embryos for Gene Editing
10.3791/56281-v
High Resolution Fluorescent In Situ Hybridization in Drosophila Embryos and Tissues Using Tyramide Signal Amplification
10.3791/56319-v
12:06 min
Generation of a Gene-disrupted Streptococcus mutans Strain Without Gene Cloning
10.3791/56728-v
10:29 min
Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput
10.3791/56752-v
Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation
10.3791/56837-v
13:03 min
Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
10.3791/56898-v
Metabolic Labeling and Profiling of Transfer RNAs Using Macroarrays
10.3791/56915-v
10:42 min
A Protocol for the Production of Integrase-deficient Lentiviral Vectors for CRISPR/Cas9-mediated Gene Knockout in Dividing Cells
10.3791/57019-v
06:11 min
Production and Purification of Baculovirus for Gene Therapy Application
10.3791/57056-v
09:59 min
Investigation of RNA Synthesis Using 5-Bromouridine Labelling and Immunoprecipitation
10.3791/57149-v
07:59 min
An Optogenetic Method to Control and Analyze Gene Expression Patterns in Cell-to-cell Interactions
10.3791/57320-v
Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions
10.3791/57471-v
12:24 min
Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens
10.3791/57494-v
06:21 min
An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness
10.3791/57518-v
08:37 min
A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins
10.3791/57772-v
12:11 min
Methodology for Accurate Detection of Mitochondrial DNA Methylation
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