Colorimetric Paper-based Detection of Escherichia coli, Salmonella spp., and Listeria monocytogenes from Large Volumes of Agricultural Water

作者： Bledar Bisha1, Jaclyn A. Adkins2, Jana C. Jokerst3, Jeffrey C. Chandler1, Alma Pérez-Méndez4, Shannon M. Coleman4, Adrian O. Sbodio5, Trevor V. Suslow5, Michelle D. Danyluk6, Charles S. Henry2, Lawrence D. Goodridge7 1Department of Animal Science,University of Wyoming, 2Department of Chemistry,Colorado State University, 3Department of Environmental and Radiological Health Sciences,Colorado State University, 4Department of Animal Sciences,Colorado State University, 5Department of Plant Sciences,University of California, Davis, 6Department of Food Science and Human Nutrition, Citrus Research and Education Center,University of Florida, 7Department of Food Science and Agricultural Chemistry,McGill University

简介：

Overview

This article presents a protocol for detecting foodborne pathogens in large volumes of agricultural water. The method involves filtering water through Modified Moore Swabs (MMS), followed by enrichment and colorimetric detection using paper-based analytical devices.

Key Study Components

Area of Science

• Microbiology
• Food Safety
• Agricultural Water Quality

Background

• Foodborne pathogens pose significant risks to public health.
• Large volumes of agricultural water can harbor these pathogens.
• Effective detection methods are crucial for ensuring water safety.
• Colorimetric detection offers a simple and effective approach.

Purpose of Study

• To develop a reliable protocol for pathogen detection in agricultural water.
• To utilize MMS for concentrating target bacteria from large water samples.
• To implement colorimetric detection for easy identification of pathogens.

Methods Used

• Assembly of MMS using gauze or cheesecloth.
• Concentration of bacteria by passing water through MMS.
• Enrichment of collected samples with selective or non-selective media.
• Incubation of samples for 18 to 24 hours before detection.

Main Results

• Successful concentration of target bacteria from large water volumes.
• Effective enrichment of cultures for pathogen detection.
• Colorimetric detection provided clear results for E. coli.
• The protocol is applicable for routine monitoring of agricultural water.

Conclusions

• The developed protocol is efficient for detecting foodborne pathogens.
• MMS and colorimetric detection are valuable tools in water safety.
• This method can enhance food safety practices in agriculture.

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