简介：
Overview
This article presents a standardized procedure for measuring total culturable viruses using the Buffalo Green Monkey kidney cell line. The method is designed to assess the presence of infectious viruses in environmental and drinking water samples.
Key Study Components
Area of Science
- Virology
- Environmental Science
- Microbiology
Background
- Measuring infectious viruses in water is crucial for public health.
- The protocol is based on EPA Method 1615.
- Challenges include contamination control and interpreting cytopathic effects (CPE).
- This method provides a standardized approach for virus detection.
Purpose of Study
- To quantify infectious virus particles in water samples.
- To provide a reliable method for assessing viral contamination.
- To support risk assessment related to waterborne viruses.
Methods Used
- Use of alkaline-based extract solution for virus elution.
- Collection of viruses on an electro-positive filter.
- Centrifugation and pH adjustment to isolate viruses.
- Inoculation of Buffalo Green Monkey kidney cells to observe CPE.
Main Results
- Successful detection of virus replication in cell cultures.
- Application of a Most Probable Number (MPN) calculator for quantification.
- Standardized results that can inform public health decisions.
- Demonstration of effective virus isolation techniques.
Conclusions
- The protocol offers a reliable method for measuring viruses in water.
- It enhances understanding of viral risks in drinking and recreational waters.
- Future applications can improve water safety assessments.
What is the main goal of this protocol?
The main goal is to measure the level of infectious virus particles in water samples.
What cell line is used in this study?
The Buffalo Green Monkey kidney cell line is used for virus detection.
How are viruses isolated from water samples?
Viruses are isolated using an alkaline-based extract solution and an electro-positive filter.
What is the significance of cytopathic effects (CPE)?
CPE indicates virus replication in the cell cultures, confirming the presence of infectious viruses.
How does this method contribute to public health?
It provides a standardized approach for assessing viral contamination in water, aiding in risk assessment.
What challenges are associated with this protocol?
Challenges include maintaining cell line contamination-free and accurately interpreting CPE.
繁體中文
