Automated Microbial Cultivation and Adaptive Evolution using Microbial Microdroplet Culture System (MMC)

作者： Xingjin Jian*1,2, Xiaojie Guo*1,2,3, Jia Wang4, Zheng Lin Tan1,2,6, Xin-hui Xing1,2,5, Liyan Wang3, Chong Zhang1,2,5 1Department of Chemical Engineering, Institute of Biochemical Engineering,Tsinghua University, 2Key Laboratory of Industrial Biocatalysis, Ministry of Education,Tsinghua University, 3Luoyang TMAXTREE Biotechnology Co., Ltd., 4Biochemical Engineering Research Group, School of Chemical Engineering and Technology,Xi’an Jiaotong University, 5Center for Synthetic & Systems Biology,Tsinghua University, 6School of Life Science and Technology,Tokyo Institute of Technology

简介：

Overview

This protocol describes the Microbial Microdroplet Culture system (MMC) for automated microbial cultivation and adaptive evolution. It offers a low-cost, user-friendly platform that enhances culture performance while reducing labor and reagent consumption.

Key Study Components

Area of Science

• Microbiology
• Automated Cultivation
• Adaptive Evolution

Background

• Conventional microbial cultivation methods can be labor-intensive and resource-consuming.
• Automated systems can improve efficiency and reliability in microbial research.
• The MMC system allows for continuous monitoring of microbial growth.
• This technology is particularly useful for growth curve measurement and adaptive laboratory evolution.

Purpose of Study

• To provide a reliable and efficient method for microbial cultivation.
• To automate the cultivation process and reduce manual labor.
• To facilitate adaptive evolution studies in microbiology.

Methods Used

• Connecting a syringe needle to a quick connector and reagent bottle.
• Utilizing the MMC system for continuous microbial culture.
• Monitoring growth online for high-throughput analysis.
• Conducting experiments with simple operational procedures.

Main Results

• The MMC system demonstrates good culture performance.
• Results show reliable outcomes with reduced labor consumption.
• Adaptive evolution can be efficiently conducted using this method.
• High throughput and parallelization are achieved in microbial studies.

Conclusions

• The MMC system is a valuable tool for microbiology research.
• It simplifies the process of microbial cultivation and evolution.
• This technology can lead to significant advancements in microbial studies.

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