Construction of a Human Aorta Smooth Muscle Cell Organ-On-A-Chip Model for Recapitulating Biomechanical Strain in the Aortic Wall

作者： Mieradilijiang Abudupataer*1, Xiujie Yin*1, Bitao Xiang*1, Nan Chen1, Shiqiang Yan2, Shichao Zhu1, Yang Ming1, Gang Liu1, Xiaonan Zhou1, Hao Lai1, Chunsheng Wang1, Kai Zhu1, Jun Li1 1Department of Cardiac Surgery and Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital,Fudan University, 2Institutes of Biomedical Sciences and the Shanghai Key Laboratory of Medical Epigenetics, Shanghai Medical College,Fudan University

简介：

Overview

This study presents a human aorta smooth muscle cell organ-on-a-chip model designed to replicate the biomechanical strain experienced by smooth muscle cells in the human aortic wall. This innovative platform allows for the simulation of mechanical stimuli and can be utilized for drug screening and personalized medicine in the context of aortic diseases.

Key Study Components

Area of Science

• Biomedical Engineering
• Cardiovascular Research
• Cellular Biology

Background

• Understanding the mechanical environment of smooth muscle cells is crucial for studying aortic diseases.
• Current models do not adequately replicate the in vivo conditions experienced by these cells.
• Patient-derived cells can enhance the relevance of in vitro studies.
• Organ-on-a-chip technology offers a novel approach to mimic physiological conditions.

Purpose of Study

• To develop a model that accurately simulates the biomechanical stimuli of smooth muscle cells in the aorta.
• To provide a platform for drug screening relevant to aortic diseases.
• To facilitate personalized medicine approaches using patient-derived cells.

Methods Used

• Harvesting smooth muscle cells from the media layer of the human aorta.
• Utilizing organ-on-a-chip technology to recreate mechanical parameters.
• Combining patient-derived cells with the organ-on-a-chip model.
• Demonstrating the procedure through a presentation by a laboratory researcher.

Main Results

• The model successfully mimics the mechanical environment of smooth muscle cells.
• It allows for precise control of mechanical parameters.
• Demonstrated potential for drug screening applications.
• Provides insights into the pathogenesis of aortic diseases.

Conclusions

• This organ-on-a-chip model represents a significant advancement in studying aortic diseases.
• It opens new avenues for personalized medicine and drug development.
• Future studies can leverage this model to further understand aortic disease mechanisms.

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