Application of the Intelligent High-Throughput Antimicrobial Sensitivity Testing/Phage Screening System and Lar Index of Antimicrobial Resistance

作者： Ming Hu1, Zhengjie Liu1,2, Ziang Song1,3, Lulu Li1, Xiaonan Zhao1, Yanbo Luo1, Qing Zhang1, Yibao Chen1, Xiaohui Xu1, Yahui Dong1, Nataliia Hrabchenko1, Wei Zhang3, Yuqing Liu1 1Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, P.R. China,Key Laboratory of Livestock and Poultry Multi-omics of MARA, 2Department of Genetic and Genomes Biology,University of Leicester, 3College of Veterinary Medicine,Nanjing Agricultural University

简介：

Overview

This protocol presents an intelligent high-throughput antimicrobial sensitivity testing/phage screening system. It illustrates its application using Salmonella isolated from poultry in Shandong, China, and discusses the significance of the Lar index in evaluating antimicrobial resistance.

Key Study Components

Area of Science

• Microbiology
• Veterinary Science
• Antimicrobial Resistance

Background

• The need for effective antimicrobial sensitivity testing.
• Challenges in monitoring pathogenic bacteria.
• Importance of high-throughput methods in research.
• Development of veterinary monitoring networks.

Purpose of Study

• To introduce a cost-effective method for antimicrobial sensitivity testing.
• To establish a system for monitoring and controlling pathogenic bacteria.
• To evaluate the significance of the Lar index in antimicrobial resistance.

Methods Used

• High-throughput antimicrobial sensitivity testing.
• Phage screening techniques.
• Calculation of the Lar index.
• Establishment of a veterinary monitoring network (VAMS).

Main Results

• Successful application of the system on Salmonella isolates.
• Collection of 20,000 epidemic genes and analytic phages.
• Demonstration of the system's effectiveness in various fields.
• Comprehensive discussion on antimicrobial resistance evaluation.

Conclusions

• The intelligent high-throughput system is effective for antimicrobial testing.
• It provides valuable insights into antimicrobial resistance.
• The method has broad applications in medicine, agriculture, and fisheries.

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