Sonodynamic Therapy for the Treatment of Glioblastoma Multiforme in a Mouse Model Using a Portable Benchtop Focused Ultrasound System

作者： Griffin Mess1,2, Taylor Anderson1,2, Shivani Kapoor3, Rasika Thombre1,2, Ruixing Liang4, Emre Derin1, Kelley M. Kempski-Leadingham1, Santosh K. Yadav5, Betty Tyler1, Amir Manbachi1,2,4,6,7 1Department of Neurosurgery,Johns Hopkins University School of Medicine, 2Department of Biomedical Engineering,Johns Hopkins University, 3Krieger School of Arts and Sciences,Johns Hopkins University, 4Department of Electrical Engineering and Computer Science,Johns Hopkins University, 5Department of Radiology, Cancer Imaging Division,Johns Hopkins University School of Medicine, 6Department of Mechanical Engineering,Johns Hopkins University, 7Department of Anesthesiology and Critical Care Medicine,Johns Hopkins University School of Medicine

简介：

Overview

This article presents a protocol for sonodynamic therapy in a mouse glioblastoma model using magnetic resonance-guided focused ultrasound. The method aims to provide a non-invasive treatment option for aggressive brain tumors.

Key Study Components

Area of Science

• Neuroscience
• Oncology
• Medical Imaging

Background

• Sonodynamic therapy is a low-intensity ultrasound treatment specifically targeting glioblastoma.
• MR guidance enhances precision in delivering ultrasound energy to tumors while protecting surrounding healthy tissue.
• This technique offers an alternative to invasive surgeries and traditional chemotherapies.
• Technical expertise is required for operating MRI and focused ultrasound systems.

Purpose of Study

• To establish a reliable protocol for preclinical sonodynamic therapy in glioblastoma research.
• To provide literature-backed parameters for effective treatment in rodent models.
• To demonstrate the potential of non-ionizing radiation in treating brain tumors.

Methods Used

• Preparation of the MRI bed and animal positioning for imaging.
• Use of MRI to guide focused ultrasound treatment.
• Application of sonication parameters to target tumor regions.
• Measurement of tumor growth rates post-treatment using luminescence.

Main Results

• Treated animals exhibited a reduction in tumor growth compared to controls.
• The protocol demonstrated repeatability and reliability for researchers.
• Guided focused ultrasound effectively targeted glioblastoma in the rodent model.

Conclusions

• Sonodynamic therapy shows promise as a non-invasive treatment for glioblastoma.
• The established protocol can aid future research in focused ultrasound applications.
• Further studies are needed to explore the full potential of this technique in clinical settings.

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