Surgical Model for Single-Staged Tissue-Engineered Urothelial Tubes in Minipigs

作者： Nikolai Juul1,2, Oliver Willacy1,2, Anastasia Buch Kjeldgaard1,2, Dennis Rootsi3, Karsten Hammelev4, Clara Ibel Chamorro3, Magdalena Fossum1,2,3 1Division of Pediatric Surgery, Department of Surgery and Transplantation,Copenhagen University Hospital - Rigshospitalet, 2Laboratory of Tissue Engineering, Department of Clinical Medicine,University of Copenhagen, 3Laboratory of Tissue Engineering, Department of Women's and Children's Health,Karolinska Institutet, 4Department of Experimental Medicine,University of Copenhagen

简介：

Overview

This study presents a novel single-staged procedure for urinary diversion using a collagen-based tubular scaffold embedded with autologous micrografts. The method aims to simplify the tissue-engineering process, which often faces challenges in preclinical trials due to complex scaffold requirements.

Key Study Components

Area of Science

• Tissue engineering
• Reconstructive surgery
• Urology

Background

• Tissue-engineered implants often fail to progress beyond preclinical trials.
• Current methods involve complex and costly scaffold components.
• There is a need for more accessible and efficient surgical procedures.
• Collagen-based scaffolds can provide a biocompatible solution for urinary diversion.

Purpose of Study

• To develop a simplified surgical procedure for urinary diversion.
• To utilize a collagen-based scaffold that incorporates autologous micrografts.
• To evaluate the feasibility and effectiveness of the new method.

Methods Used

• Preparation of a collagen-based scaffold with minced mucosal micrografts.
• Standard surgical techniques for bladder segment excision and scaffold implantation.
• Post-operative evaluation through endoscopy and CT imaging.
• Histological analysis of the scaffold tissue after six weeks.

Main Results

• The tubular scaffold remained patent and unobstructed after six weeks.
• No signs of host rejection or infection were observed.
• Histological evaluations showed a stratified luminal epithelium of urothelial origin.
• Remnants of the reinforcing biomaterials were still visible after six weeks.

Conclusions

• The single-staged procedure is a viable alternative for urinary diversion.
• Collagen-based scaffolds with autologous micrografts can enhance surgical outcomes.
• This method may facilitate the transition of tissue-engineered implants into clinical practice.

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