Translating Extracellular Electron Transfer Activities with Organic Electrochemical Transistors

作者： Yang Gao1, Yuchen Zhou2,3, Xudong Ji4,5, Austin J. Graham1,6, Christopher M. Dundas1,7, Ismar E. Miniel Mahfoud1, Bailey M. Tibbett1, Benjamin Tan3,8, Gina Partipilo1, Ananth Dodabalapur2,3, Jonathan Rivnay4,5, Benjamin K. Keitz1 1McKetta Department of Chemical Engineering,University of Texas at Austin, 2Department of Electrical and Computer Engineering,The University of Texas at Austin, 3Microelectronics Research Center,The University of Texas at Austin, 4Department of Biomedical Engineering,Northwestern University, 5Simpson Querrey Institute,Northwestern University, 6Department of Pharmaceutical Chemistry,University of California San Francisco, 7Department of Biology,Stanford University, 8Department of Chemistry,University of Texas at Austin

简介：

Overview

This study presents a protocol for utilizing organic electrochemical transistors (OECTs) to convert extracellular electron transfer (EET) activity in Shewanella oneidensis into measurable electrical signals. The hybrid OECT system enhances robustness and sensitivity, facilitating rapid and high-throughput testing for EET measurements.

Key Study Components

Area of Science

• Bioelectronics
• Extracellular electron transfer
• Electrochemical systems

Background

• Research focuses on integrating bacterial EET with electronic materials.
• Exploration of genetic regulation of EET for improved electrical performance.
• Investigation of emergent features in electrochemical systems with living cells.
• Use of synthetic biology to engineer EET pathways.

Purpose of Study

• To develop bioelectronics that leverage EET for biosensing and biocomputing.
• To understand the interaction between EET and electronic materials.
• To optimize electrical performance through genetic modulation of EET.

Methods Used

• Engineering of bacterial cells to modulate OECT outputs.
• Electrochemical systems for redox monitoring.
• Microscopy techniques for analyzing cell activities.
• Advanced spectroscopy methods for characterizing material-biology interfaces.

Main Results

• Demonstrated that genetically engineered bacteria can influence OECT performance.
• Illustrated direct and indirect EET pathways affecting bioelectronics.
• Coupled genetic logic to electrical outputs for enhanced control.
• Showed advantages of using living cells for dynamic responses in bioelectronics.

Conclusions

• Living cells provide programmable controls through EET.
• Cellular metabolism can be leveraged for real-time electrical responses.
• Findings contribute to the advancement of bioelectronic applications.

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