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In Vitro Resident Memory CD8 T Cell Differentiation Using Epithelial Organoid-T Cell Co-culture System

作者： Ying Lin*1,2, Julian C. Ramprashad*1, Elizabeth L. Hillier1,2, Lalit K. Beura1 1Department of Molecular Microbiology and Immunology,Brown University, 2Pathobiology Graduate Program,Brown University
简介：

To study CD8 T cell differentiation and function in vitro, CD8 T cells can be isolated from the mouse to be co-cultured long-term with preformed infected murine vaginal epithelial organoids. Here, we describe this process and assess the acquisition of resident memory T cell markers upon co-culture.

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