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A Multiplex Serological Assay for the Detection of Antibody Responses to Arboviruses

作者： Marie-Fabrice Gasasira1, Laura Garcia1, Françoise Donnadieu1, Stéphane Pelleau1, Jessica Vanhomwegen2, Lova Tsikiniaina Rasoloharimanana3, Ilaria Dorigatti4, Matthieu Schoenhals3,7, Aissatou Toure-Balde5, Inès Vigan-Womas5,6, Makhtar Niang5, Michael White1 1Infectious Diseases Epidemiology and Analytics G5 Unit, Department of Global Health,Institut Pasteur, Université Paris-Cité, INSERM U1347, 2Laboratory for Urgent Response to Biological Threats (CIBU), Environment and Infectious Risks (ERI) Unit,Institut Pasteur, Université Paris Cité, 3Immunology of Infectious Diseases Unit,Institut Pasteur de Madagascar, 4MRC Centre for Infectious Disease Analysis and Modelling, School of Public Health,Imperial College London, 5Immunophysiopathology and Infectious Diseases Department,Institut Pasteur de Dakar, 6Pasteur Network,Institut Pasteur, 7Department of Immunology,Institut Pasteur
简介：

Multiplex fluorescence immunoassays are becoming a reference for serology testing. Their application, however, is limited by the complexity and time-consuming bead-analyte coupling processes. This report presents a semi-automated, reproducible multiplex microsphere bead coupling protocol that is comparable to the standard method while considerably reducing time to results.

标签: Immunology and Infection,   
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