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JoVE Encyclopedia of Experiments
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癌症
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10.3791/51998-v
08:01 min
The Soft Agar Colony Formation Assay
10.3791/50762-v
Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology
10.3791/51295-v
12:03 min
Expression, Isolation, and Purification of Soluble and Insoluble Biotinylated Proteins for Nerve Tissue Regeneration
10.3791/1444-v
Obtaining High Quality RNA from Single Cell Populations in Human Postmortem Brain Tissue
10.3791/3702-v
In vitro Transcription and Capping of Gaussia Luciferase mRNA Followed by HeLa Cell Transfection
10.3791/59218-v
11:48 min
Generation, Amplification, and Titration of Recombinant Respiratory Syncytial Viruses
10.3791/59626-v
08:58 min
In Vitro Transcribed RNA-based Luciferase Reporter Assay to Study Translation Regulation in Poxvirus-infected Cells
10.3791/56844-v
08:22 min
CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy
10.3791/57278-v
Highly Efficient Gene Disruption of Murine and Human Hematopoietic Progenitor Cells by CRISPR/Cas9
10.3791/53245-v
09:55 min
From Constructs to Crystals – Towards Structure Determination of β-barrel Outer Membrane Proteins
10.3791/55187-v
10:09 min
Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX
10.3791/2099-v
07:56 min
Bacterial Delivery of RNAi Effectors: Transkingdom RNAi
10.3791/60829-v
09:37 min
Preparing Protein Producing Synthetic Cells using Cell Free Bacterial Extracts, Liposomes and Emulsion Transfer
10.3791/58165-v
05:42 min
Quantitative PCR of T7 Bacteriophage from Biopanning
10.3791/61373-v
10:28 min
A Cell Culture Model for Producing High Titer Hepatitis E Virus Stocks
10.3791/61039-v
11:40 min
Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins
10.3791/59333-v
12:22 min
A Strategy to Identify Compounds that Affect Cell Growth and Survival in Cultured Mammalian Cells at Low-to-Moderate Throughput
10.3791/55312-v
08:14 min
Kinetics of Lagging-strand DNA Synthesis In Vitro by the Bacteriophage T7 Replication Proteins
10.3791/59635-v
11:34 min
Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
10.3791/57816-v
11:49 min
A Novel Saturation Mutagenesis Approach: Single Step Characterization of Regulatory Protein Binding Sites in RNA Using Phosphorothioates
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