An IL-8 Transiently Transgenized Mouse Model for the In Vivo Long-term Monitoring of Inflammatory Responses

作者： Gabriella Bergamini*1, Fabio Stellari*2, Angela Sandri*3, Maria M. Lleo3, Gaetano Donofrio4, Francesca Ruscitti5,2, Federico Boschi6, Andrea Sbarbati7, Gino Villetti2, Paola Melotti8, Claudio Sorio1 1Department of Medicine, General Pathology Division, Cystic Fibrosis Translational Research Laboratory "D. Lissandrini",University of Verona, 2Corporate Preclinical R&D,Chiesi Farmaceutici S.p.A., 3Department of Diagnostic and Public Health, Microbiology Division,University of Verona, 4Dipartimento di Scienze Medico-Veterinarie,University of Parma, 5Department of Biomedical Biotechnological and Translational Sciences,University of Parma, 6Department of Computer Science,University of Verona, 7Department of Neurological, Biomedical and Movement Sciences,University of Verona, 8Cystic Fibrosis Regional Center (CFC),AOUI Verona

简介：

Overview

This method enables the visualization of IL-8 promoter-dependent inflammation activation in the lungs of mice using non-invasive bioluminescence imaging (BLI). It allows for repeated monitoring of the same animals over a two-month period following the delivery of the luciferase reporter construct.

Key Study Components

Area of Science

• Neuroscience
• Immunology
• In vivo imaging

Background

• Real-time monitoring of lung inflammation is crucial for understanding respiratory diseases.
• Non-invasive techniques provide significant advantages in longitudinal studies.
• Host-pathogen interactions can be better understood through this method.
• Visual demonstrations aid in the learning of complex installation steps.

Purpose of Study

• To monitor lung inflammation in mice after exposure to pro-inflammatory stimuli.
• To identify bacterial products responsible for respiratory infections.
• To demonstrate a non-invasive imaging technique for repeated assessments.

Methods Used

• Non-invasive bioluminescence imaging (BLI).
• Delivery of luciferase reporter constructs.
• Monitoring of inflammation over time in the same animals.
• Use of bacterial culture supernatants as a pro-inflammatory stimulus.

Main Results

• Successful visualization of IL-8 promoter activity in lung inflammation.
• Ability to track changes in inflammation over a two-month period.
• Demonstrated the feasibility of using the same animals for multiple assessments.
• Provided insights into the dynamics of host-pathogen interactions.

Conclusions

• This method is effective for studying lung inflammation in real-time.
• Non-invasive imaging techniques enhance the understanding of respiratory diseases.
• Future studies can leverage this approach for deeper insights into inflammation mechanisms.

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