Preparation of Mesh-Shaped Engineered Cardiac Tissues Derived from Human iPS Cells for In Vivo Myocardial Repair

作者： Takeichiro Nakane1,2,6, Mosha Abulaiti1,2, Yuko Sasaki1, William J. Kowalski3, Bradley B. Keller4,5,7, Hidetoshi Masumoto1,2 1Clinical Translational Research Program,RIKEN Center for Biosystems Dynamics Research, 2Department of Cardiovascular Surgery, Graduate School of Medicine,Kyoto University, 3Laboratory of Stem Cell and Neuro-Vascular Biology,Cell and Developmental Biology Center, National Institutes of Health, 4Kosair Charities Pediatric Heart Research Program, Cardiovascular Innovation Institute,University of Louisville, 5Department of Pediatrics, School of Medicine,University of Louisville, 6Department of Cardiovascular Surgery,Mitsubishi Kyoto Hospital, 7Cincinnati Children's Heart Institute

简介：

Overview

This protocol describes a novel and easy method for creating mesh-shaped engineered cardiac tissues derived from human induced pluripotent stem cells. The technique allows for the investigation of cell implantation therapy for heart diseases, particularly in a rat myocardial infarction model.

Key Study Components

Area of Science

• Cardiac tissue engineering
• Stem cell therapy
• Regenerative medicine

Background

• Engineered cardiac tissues can restore cardiac structure and function.
• Human induced pluripotent stem cells are a valuable resource for cardiac cell derivation.
• The flexibility of tissue geometry is crucial for effective implantation.
• Reproducibility in stem cell processing is essential for consistent results.

Purpose of Study

• To develop a method for creating mesh-shaped engineered cardiac tissues.
• To evaluate the feasibility of these tissues in cardiac regenerative therapy.
• To investigate the restoration of cardiac function post-implantation.

Methods Used

• Fabrication of a rectangular tray using PDMS and silicone adhesive.
• Creation of posts for supporting the engineered tissues.
• Implantation of the engineered cardiac tissues in a rat model.
• Assessment of cardiac structure and function restoration.

Main Results

• The mesh-shaped engineered cardiac tissues successfully restored cardiac function.
• The method demonstrated scalability and flexibility in tissue geometry.
• Consistent results were achieved through reproducible stem cell processing.
• The technique is feasible for use in cardiac regenerative therapy.

Conclusions

• This protocol provides a reliable method for creating engineered cardiac tissues.
• Mesh-shaped tissues can effectively restore cardiac function in a myocardial infarction model.
• The approach holds promise for future cardiac regenerative therapies.

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