Portable Paper-Based Immunoassay Combined with Smartphone Application for Colorimetric and Quantitative Detection of Dengue NS1 Antigen

作者： Muhammad Hatta Prabowo1,2, Thanit Chalermwatanachai3, Werasak Surareungchai2, Patsamon Rijiravanich4,5 1Department of Pharmacy, Faculty of Mathematics and Natural Sciences,Universitas Islam Indonesia, 2School of Bioresources and Technology,King Mongkut's University of Technology Thonburi, 3Department of Otolaryngology,Phramongkutklao Hospital and College of Medicine, 4Sensor Technology Laboratory, Pilot Plant Development and Training Institute,King Mongkut's University of Technology Thonburi, 5Biosciences and Systems Biology Research Team, National Center for Genetic Engineering and Biotechnology,National Sciences and Technology Development Agency at King Mongkut's University of Technology Thonburi

简介：

Overview

This study presents a smartphone app-integrated Dengue NS1 Paper-based Analytical Device (DEN-NS1-PAD) designed for quantifying Dengue NS1 antigen concentration in clinical serum samples. The device aims to enhance dengue management by improving clinical decision-making, particularly in resource-limited healthcare settings.

Key Study Components

Area of Science

• Neuroscience
• Biotechnology
• Diagnostic Devices

Background

• Dengue fever is a significant global health concern.
• Current diagnostic methods may be inadequate in resource-limited settings.
• NS1 antigen is a critical biomarker for dengue detection.
• There is a need for simple, cost-effective diagnostic solutions.

Purpose of Study

• To develop a sensitive and specific device for dengue detection.
• To integrate a smartphone application to enhance measurement accuracy.
• To provide scalable solutions for dengue diagnostics.

Methods Used

• Development of a paper-based analytical device (PAD).
• Fabrication of microfluidic methods based on PEC.
• Integration of a smartphone application for enhanced sensitivity.
• Testing the device with real serum samples.

Main Results

• The DEN-NS1-PAD demonstrated improved sensitivity in detecting NS1.
• The smartphone application significantly enhanced measurement efficiency.
• Results showed better performance compared to traditional observation methods.
• The device is suitable for use in various healthcare settings.

Conclusions

• The DEN-NS1-PAD is a promising tool for dengue diagnostics.
• Integration with mobile technology offers significant advantages.
• This innovation can improve clinical decision-making in dengue management.

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