Preparing a Mice Model of Severe Acute Pancreatitis via a Combination of Caerulein and Lipopolysaccharide Intraperitoneal Injection

作者： Lei Xu*1,2, Manman Xu*3, Yinghai Xie4, Wei You2, Jianxin Wang2, Liwu Xu4, Qizhu Feng4, Jie Sun4, Jian Zhang4, Huaichen Yang4, Wang Qi4 1The First Affiliated Hospital of Anhui University of Science & Technology, 2Medical College of Anhui University of Science & Technology, 3Endocrinology Department,The First Affiliated Hospital of Anhui University of Science & Technology, 4Department of Hepatobiliary Surgery,The First Affiliated Hospital of Anhui University of Science & Technology

简介：

Overview

This study investigates severe acute pancreatitis (SAP) using a non-invasive intraperitoneal drug administration method in mice. By comparing five injection protocols, the research establishes a model for inducing varying degrees of pancreatic injury, aiming to uncover therapeutic targets for SAP.

Key Study Components

Area of Science

• Neuroscience
• Biology
• Pathology

Background

• Severe acute pancreatitis is associated with high mortality rates.
• Animal models, particularly C57BL/6J mice, are commonly used for research.
• Understanding SAP pathology is crucial for developing new treatments.
• HMGB1 gene expression is linked to inflammation in SAP.

Purpose of Study

• To establish a reliable model for severe pancreatic injury.
• To investigate pathological changes associated with SAP.
• To identify potential therapeutic targets for treatment strategies.

Methods Used

• Intraperitoneal administration of Caerulein to induce gall bladder contraction.
• Disruption of fasting in mice to promote pancreatic tissue damage.
• Combination of Caerulein with LPS injection to induce severe pancreatitis.
• Assessment of pancreatic injury and organ failure in the model.

Main Results

• Successful induction of severe pancreatitis with organ failure in mice.
• High expression of HMGB1 genes observed in injured pancreatitis.
• Non-invasive method demonstrated high repeatability and ease of implementation.
• Pathological changes were effectively monitored in the established model.

Conclusions

• The intraperitoneal injection method is effective for inducing SAP in mice.
• This model can be utilized for further research on therapeutic strategies.
• Findings contribute to understanding the inflammatory mechanisms in SAP.

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