An Obstructive Chronic Pancreatitis Model Established Through Electrocoagulation

作者： Zhenghui Yang*1,2, Wanshun Li*1,2,3, Jiayu Li*1,2,3, Yuyan Zhou1,2, Chang Wu1,2, Yue Liu1,2, Xinyue Wang1,2, Dongling Wan1,2, Yi Lu1,2,3, Yihai Shi4, Huanhai Xu5, Zhendong Jin1,2, Deyu Zhang1,2, Haojie Huang1,2 1Department of Gastroenterology, Changhai Hospital,Naval Medical University, 2National Key Laboratory of Immunity and Inflammation,Naval Medical University, 3College of Basic Medical Science,Naval Medical University, 4Department of Gastroenterology,Shanghai Pudong New Area Gongli Hospital Shanghai, 5Department of Gastroenterology. Affiliated Yueqing Hospital,Wenzhou Medical University

简介：

Overview

This protocol outlines a novel method for modeling chronic obstructive pancreatitis in mice through electrocoagulation of the pancreatic duct. This approach simplifies the process of inducing chronic pancreatitis compared to traditional methods.

Key Study Components

Area of Science

• Neuroscience
• Biology
• Chronic Pancreatitis Research

Background

• Chronic pancreatitis is a debilitating condition with limited effective modeling methods.
• Traditional models often require complex procedures or long-term drug administration.
• Electrocoagulation presents a less invasive alternative for inducing chronic pancreatitis.
• This study aims to refine the model for better reproducibility and ease of use.

Purpose of Study

• To develop a reliable electrocoagulation model for chronic pancreatitis in mice.
• To evaluate the pathological changes in pancreatic tissue post-electrocoagulation.
• To assess the biochemical markers associated with chronic pancreatitis.

Methods Used

• Electrocoagulation of the pancreatic duct in anesthetized mice.
• Infusion of methylene blue to visualize the pancreatic duct.
• Histological analysis of pancreatic tissue using hematoxylin-eosin and Masson's staining.
• Measurement of serum amylase, bilirubin, and hyaluronic acid levels.

Main Results

• Histological evidence of chronic pancreatitis was observed in electrocoagulated mice.
• Increased fibrosis was noted compared to the sham group.
• Biochemical markers showed significant changes, indicating the severity of the condition.
• Recovery signs in pancreatic acinar cells were noted over time.

Conclusions

• The electrocoagulation model effectively induces chronic pancreatitis in mice.
• This method offers a simpler and more reproducible approach for future research.
• Findings contribute to understanding the pathophysiology of chronic pancreatitis.

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